Purpose of the study:

Interaction study human bioenvironment with nutrient media.

Test samples:

1. Fasting saliva sample from 03/06/2023.

2. Nutrient medium (raw pine nuts) combined with human biological medium (saliva) from 03/06/2023.

3. Fasting saliva sample from 03/12/2023.

4. Nutrient medium (raw pine nuts) combined with human biological medium (saliva) from 03/12/2023.

Evaluation was performed immediately after sample collection, after 5h, 1d, 2d, 3d and 4d.

Samples No. 1 and No. 2 were tested additionally for 5 days.

Sample #1:

Description	Photo
06.03.2023 10:30   The saliva sample contains many small and medium sized bubbles grouped together. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high.
06.03.2023 16:00   There is a combination of bubbles into one big one.
07.03.2023 13:30  The dynamics of the bubbles merging into one big one continues. An increase in the number of bacteria is observed.
08.03.2023 14:00  The dynamics of the bubbles merging into one big one continues.
09.03.2023 13:00  The dynamics of the bubbles merging into one big one continues.
10.03.2023 18:00  The dynamics of the merging of bubbles into one big one continues.
11.03.2023 13:00  The dynamics of growth in the number of bacteria continues.

Sample #2.

Description	Photo
06.03.2023 11:30 The culture medium sample combined with saliva contains small and medium sized bubbles grouped together. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high.
06.03.2023 16:00 The process of coalescence of bubbles into one large one is observed.
07.03.2023 13:30 The dynamics of the bubbles merging into one big one continues.
08.03.2023 14:00 The dynamics of the bubbles merging into one big one continues.
09.03.2023 13:00 The dynamics of the bubbles merging into one big one continues. Bacterial growth is observed.
10.03.2023 18:00  There is an increase in tension.
11.03.2023 13:00  The dynamics of voltage growth continues.

Sample #3:

Description	Photo
12.03.2023 13:00  The saliva sample contains many small and medium sized bubbles grouped together. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high. A precipitate is immediately present.
12.03.2023 19:00   There is a combination of bubbles into one big one.
13.03.2023 12:00  The dynamics of the bubbles merging into one big one continues.
14.03.2023 15:00  The dynamics of the bubbles merging into one big one continues.
15.03.2023 09:00  There is a release of tension – a large bubble has burst.
16.03.2023 10:00  The dynamics of stress relief continues – there are almost no bubbles. The liquid has become more uniform.

Sample #4:

Description	Photo
12.03.2023 15:00  A sample of culture medium combined with saliva contains a few small bubbles not grouped. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are low.
12.03.2023 19:00   No changes.
13.03.2023 12:00  There is an increase in the size of the bubbles.
14.03.2023 15:00  The bubble size continues to grow. Bubble convergence is observed.
15.03.2023 09:00  The process of coalescence of bubbles into one large one is observed.
16.03.2023 10:00  The dynamics of the bubbles merging into one big one continues. There is an increase in tension.

Result:

When evaluating the interaction of the above media in sample No. 4, no colonies were detected, growth was not observed.

In sample No. 2, colonies collected in a chain were immediately detected. An increase in the number of bacteria was observed on the 4th day of observation. A gradual increase in voltage was observed on all days of observation.

In a separate sample of the human bioenvironment, bacteria were initially observed in sample No. 3, and growth on the 1st day in sample No. 1, which increased on the fifth day.