Purpose of the study:
Interaction study human bioenvironment with nutrient media.
Test samples:
1. Fasting saliva sample from 02/22/2023.
2. Nutrient medium (peeled mung bean sprouts) combined with human biological medium (saliva) from 02/22/2023.
3. Nutrient medium (mung bean seedlings with peel) combined with human biological environment (saliva) dated 22.02.2023.
4. Fasting saliva sample from 03/06/2023.
5. Nutrient medium (boiled mung bean) combined with human biological environment (saliva) from 03/06/2023.
The evaluation was carried out immediately after the collection of samples, after 4-5 hours, 1d, 2d, 3d and 5d.
Samples No. 4 and No. 5 were tested additionally on the 4th day.
Sample №1:
| Description | Photo |
| 22.02.2023 6:00 The saliva sample contains many small and medium sized bubbles grouped together. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high. |  |
| 02/22/2023 10:30 Bubbles coalesce. |  |
| 02/23/2023 7:00 am Continues the dynamics of the association of bubbles into one big one. |  |
| 02/24/2023 7:00 AM Bubbles continue to merge into one big one. Most of the bubbles burst. |  |
| 02/25/2023 7:00 am Continues the dynamics of the combination of bubbles into one big burst. The process of sample drying is observed. |  |
| 02/27/2023 9:30 am Continues the dynamics of the combination of bubbles into one big burst. The drying process of the sample is completed. |  |
Sample #2.
| Description | Photo |
| 22.02.2023 6:30 The sample contains many bubbles of medium and small size combined into a group. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high.The sample is already very thick. |  |
| 22.02.2023 10:30 There is an association of bubbles and an increase in the content of bacteria. |  |
| 23.02.2023 7:00 Bubbles are practically absent. The rest are separate from each other. |  |
| 24.02.2023 7:00 The aggregation of the remaining bubbles is observed |  |
| 25.02.2023 7:00 The process of sample drying is observed. |  |
| 27.02.2023 9:30 The drying process of the sample is completed. |  |
Sample #3.
| Description | Photo |
| 22.02.2023 6:30 The sample contains many bubbles of all sizes grouped together. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high.The sample is already very thick. |  |
| 22.02.2023 10:30 An increase in the content of bacteria is observed. |  |
| 23.02.2023 7:00 Large bubbles have not changed, small ones disappear. The growth dynamics of microorganisms continues. |  |
| 24.02.2023 7:00 Large bubbles have not changed, small ones have disappeared. |  |
| 25.02.2023 7:00 The sample thickened and dried. |  |
| 27.02.2023 9:30 No changes |  |
Sample #4:
| Description | Photo |
| 06.03.2023 10:30 The saliva sample contains many small and medium sized bubbles grouped together. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high. |  |
| 06.03.2023 16:00 There is a combination of bubbles into one big one. |  |
| 07.03.2023 13:30 The dynamics of the bubbles merging into one big one continues. An increase in the number of bacteria is observed. |  |
| 08.03.2023 14:00 The dynamics of the bubbles merging into one big one continues. |  |
| 09.03.2023 13:00 The dynamics of the bubbles merging into one big one continues. |  |
| 10.03.2023 18:00 The dynamics of the merging of bubbles into one big one continues. |  |
| 11.03.2023 13:00 The dynamics of growth in the number of bacteria continues. |  |
Sample number 5.
| Description | Photo |
| 06.03.2023 11:30 A sample of culture medium combined with saliva contains bubbles of all sizes combined in a group. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high. |  |
| 06.03.2023 16:00 There is a process of combining bubbles into one large |  |
| 07.03.2023 13:30 The dynamics of the bubbles merging into one big one continues. |  |
| 08.03.2023 14:00 The big bubble burst. Relaxation of tension. |  |
| 09.03.2023 13:00 The remaining bubbles are combined into one big one. There is a slight turbidity of the liquid. |  |
| 10.03.2023 18:00 No changes. |  |
| 11.03.2023 13:00 No changes. |  |
Result:
When assessing the interaction of the above media in samples No. 2 and No. 3, colonies collected in chains were immediately identified, which already at 4 hours of observation showed growth of microorganisms in samples No. 3 and No. 4. Samples No. 3 and No. 4 were immediately thick, while in both samples the biofilm voltage surface and the electrical activity of bacteria noticeably decreased every day, and the groups of colonies disintegrated.
In sample No. 5, the voltage decreased on the 2nd day of observation.
In a separate sample, the human bioenvironment did not show growth in sample No. 1, but in sample No. 4 – heighton the 1st day, which intensified on the fifth day.
Conclusion:
The researcher should completely exclude mung beans in any form from the diet.
There is no need for retesting.