The purpose of the study:
The study of the interaction of human biological media with nutrient media.
Test samples:
- Sample of saliva on an empty stomach.
- Nutrient medium (raw hazelnuts) combined with human biological medium (saliva) from 08.02.2023.
- Sample of saliva on an empty stomach from 13.02.2023.
4-5. Nutrient medium (raw hazelnuts) combined with human bio-environment (saliva) from 02/13/2023.
Samples №1 and №2 were evaluated immediately after their collection, after 4h, 1d, 2d, 3d and 4d.
Additionally, sample №2 was studied on the 6th day.
The evaluation of samples №3-5 was carried out immediately after collection, after 4-5h, 1d, 2d and 5d.
Sample №1:
| Description | Photo |
| 08.02.2023 16:00 The saliva sample contains many medium-sized bubbles grouped together. According to the size and nature, it can be concluded that the surface voltage of the biofilm and the electrical activity of the bacteria that make up the biofilm are high. |  |
| 08.02.2023 20:00 There is a union of bubbles. |  |
| 09.02.2023 16:30 The dynamics of combining bubbles into one big one continues. |  |
| 10.02.2023 22:00 The liquid is almost absent as a result of drying of the sample. |  |
| 11.02.2023 20:00 The dynamics of an increase in a large bubble continues. |  |
| 12.02.2023 17:00 Complete drying of the sample. There is no need for further observation. |  |
Sample №2.
| Description | Photo |
| 08.02.2023 16:30 A sample of a nutrient medium combined with saliva contains a small number of small and medium-sized bubbles combined into a group. According to the size and nature, it can be concluded that the surface voltage of the biofilm and the electrical activity of the bacteria that make up the biofilm is average. |  |
| 08.02.2023 20:30 There is a union of bubbles. |  |
| 09.02.2023 16:30 There is a union of bubbles into one large one. There are almost no chains. |  |
| 10.02.2023 22:00 The dynamics of combining bubbles into one big one continues. |  |
| 11.02.2023 20:00 All the bubbles burst |  |
| 12.02.2023 03:30 There are no changes |  |
| 14.02.2023 3:00 Obviously, the dynamics of combining bubbles into one large one continued. The bubble has burst, which means a weakening of the stress surface. There is a turbidity of the liquid, but the fluidity is very good. The liquid turned orange. |  |
Sample №3.
| Description | Photo |
| 13.02.2023 0:00 The saliva sample contains many small bubbles grouped together. According to the size and nature, it can be concluded that the surface voltage of the biofilm and the electrical activity of the bacteria that make up the biofilm are high. |  |
| 13.02.2023 5:30 There is a union of bubbles into one big one. |  |
| 13.02.2023 21:30 The dynamics of combining bubbles into one big one continues. |  |
| 14.02.2023 23:00 The dynamics of combining bubbles into one big one continues. | Photo lost |
| 17.02.2023 23:00 The dynamics of combining bubbles into one big one continues. There is an increase in the number of bacteria. |  |
Sample №4 и №5.
| Description | Photo |
| 13.02.2023 2:00 Samples of the nutrient medium combined with saliva contain a small number of small bubbles combined in groups. By its size and nature, it can be concluded that the surface voltage of the biofilm and the electrical activity of the bacteria that make up the biofilm are below average. |  |
| 13.02.2023 5:30 There are no changes |  |
| 13.02.2023 21:30 There is a disconnection of circuits, the union of bubbles into large ones and a voltage reset (bubbles burst) |  |
| 14.02.2023 23:00 The dynamics of combining bubbles and reducing stress continues. |  |
| 17.02.2023 23:00 Sample №4 has shrunk. In sample №5, the liquid moves freely. |  |
Result:
When evaluating the interaction of the two above-mentioned media in sample №2, colonies were immediately identified, collected in chains, which, when observed, merged into one large bubble, which burst between 4 and 6 days of observation. On day 6, a change in the color of the nutrient medium was observed.
When assessing the interaction of biological media in samples №4 and №5, small colonies were immediately identified, collected in chains, which opened on the same day of observation. There was also a decrease in tension and the absence of bacterial growth.
In a separate sample of the human biological environment in sample №1, growth was not shown, in sample №3, growth was observed on the 5th day of observation.
Conclusion:
The researcher’s use of raw hazelnuts in the diet is acceptable.