Purpose of the study:

Interaction study human bioenvironment with nutrient media.

Test samples:

1. Fasting saliva testfrom 08.02.2023.

2. Nutrient medium (in equal proportions cucumber juice + distilled water) combined with the human biological environment (saliva).

3. Fasting saliva sample dated February 13, 2023.

4. Nutrient medium (raw cucumber) combined with human biological medium (saliva).

The evaluation was carried out immediately after the collection of samples, after 4-5 hours, 1d, 2d.

Additionally, sample No. 2 was studied on the 6th day,No. 1 of3d and 4d, #3 and #4 on 5d.

Sample №1:

Description	Photo
08.02.2023 16:00 The saliva sample contains many medium-sized bubbles grouped together. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high.
08.02.2023 20:00 There is an association of bubbles.
09.02.2023 16:30 The dynamics of the bubbles merging into one big one continues.
10.02.2023 22:00 The liquid is almost absent as a result of the drying of the sample.
11.02.2023 20:00 The dynamics of the increase in the large bubble continues.
12.02.2023 17:00  Complete drying of the sample. There is no need for further observation.

Sample #2.

Description	Photo
08.02.2023 16:00 The culture medium sample combined with saliva contains many small and medium sized bubbles grouped together. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high.
08.02.2023 20:00 Some bubbles burst. The appearance of small groups consisting of small bubbles is observed, separately from the main group.
09.02.2023 16:30 There is a combination of bubbles into one big one. Small bubbles form a very dense chain.
10.02.2023 22:00 The dynamics of the bubbles merging into one big one continues.
11.02.2023 20:00 The dynamics of the bubbles merging into one big one continues.
12.02.2023 17:00 The dynamics of the bubbles merging into one big one continues.
14.02.2023 3:00 It is obvious that the dynamics of the bubbles merging into one large one continued. The bubble has burst, which means the weakening of the stress surface. Cloudiness of the liquid is observed, but fluidity is very good.

Sample #3:

Description	Photo
13.02.2023 0:00 The saliva sample contains many small bubbles grouped together. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high.
13.02.2023 5:30 There is a combination of bubbles into one big one.
13.02.2023 21:30  The dynamics of the bubbles merging into one big one continues.
14.02.2023 23:00 The dynamics of the bubbles merging into one big one continues.	Photo lost
17.02.2023 23:00 The dynamics of the bubbles merging into one big one continues. An increase in the number of bacteria is observed.

Sample #4:

Description	Photo
13.02.2023 2:30 A sample of culture medium combined with saliva, contains many bubbles of small size united in a group. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high.
13.02.2023 6:00 Bubbles coalesceinto larger. Liquid does not flow.
13.02.2023 21:30  There is a separation of the group and an increase in tension.
14.02.2023 23:00 Dynamic continuesseparation of groups and rising tension.
17.02.2023 23:00 Observedaggregation of bubbles. The sample dried up. The tension didn’t subside.

Conclusion:

When evaluating sample #2, colonies collected in chains were immediately identified, which, when observed, combined into one large bubble that burst between 4 and 6 days of observation. On day 6, a lighter-than-liquid precipitate was observed, indicating the appearance of mold or grease.

When assessing sample No. 4, colonies were immediately identified, collected in a very dense chain, which completely prevented the movement of fluid. The chain under observation broke on the same day.

In a separate sample, the human bioenvironment in sample No. 1 did not show growth; in sample No. 3, growth was observed on the 5th day of observation.

Conclusion:

The use of cucumber juice in the diet by the researcher is undesirable, but not prohibited.

Raw cucumber must be retested. Preliminary – the researcher should limit the use of raw cucumber in the diet.