Purpose of the study:

Interaction study human bioenvironment with nutrient media.

Test samples:

1. Fasting saliva sample from 03/12/2023.

2. Nutrient medium (raw coconut) combined with human biological medium (saliva) from 03/12/2023.

3. Nutrient medium (coconut water) combined with human biological medium (saliva) from 03/12/2023.

Evaluation was performed immediately after sample collection, after 5h, 1d, 2d, 3d and 4d.

Sample #1:

Description	Photo
12.03.2023 13:00  The saliva sample contains many small and medium sized bubbles grouped together. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high. A precipitate is immediately present.
12.03.2023 19:00   There is a combination of bubbles into one big one.
13.03.2023 12:00  The dynamics of the bubbles merging into one big one continues.
14.03.2023 15:00  The dynamics of the bubbles merging into one big one continues.
15.03.2023 09:00  There is a release of tension – a large bubble has burst.
16.03.2023 10:00  The dynamics of stress relief continues – there are almost no bubbles. The liquid has become more uniform.

Sample #2:

Description	Photo
12.03.2023 15:00  A sample of culture medium combined with saliva contains 1 medium sized vial and several small ungrouped vials. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are low.
12.03.2023 19:00   There is a combination of bubbles into one big one.
13.03.2023 12:00  The dynamics of the bubbles merging into one big one continues.
14.03.2023 15:00  The dynamics of the bubbles merging into one big one continues.
15.03.2023 09:00  The dynamics of the bubbles merging into one big one continues. There is an increase in voltage.
16.03.2023 10:00  The dynamics of the bubbles merging into one big one and increasing the voltage continues.

Sample #3:

Description	Photo
12.03.2023 20:00  The culture medium sample combined with saliva contains 2 medium sized vials and several small ungrouped vials. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are low.
13.03.2023 00:00   There is a combination of bubbles into one big one.
13.03.2023 12:00  There is an increase in the number of small bubbles, the process of combining them into a group, as well as an increase in the number of bacteria.
14.03.2023 15:00  Grouping of bubbles into a group is completed. The dynamics of the bubbles merging into one big one continues.
15.03.2023 09:00  The dynamics of the bubbles merging into one big one continues. There is a separation of groups.
16.03.2023 10:00  The dynamics of the bubbles merging into one big one continues.There is an increase in voltage.

Conclusion:

When evaluating the interaction of the above media in sample No. 1 throughout the entire observation period, no colonies were detected and no growth was observed.

In sample No. 2, no colonies were found on the first day, thensmall bubblesstarted showing up (day 0), forming groups(day 1-2) and unite(day 3-4).

Bacteria were initially observed in a separate sample of the human bioenvironment.