The purpose of the study:
The study of the interaction of human biological media with nutrient media.
Test samples:
- Sample of saliva on an empty stomach from 13.02.2023.
- Nutrient medium (hass raw avocado) combined with human bio-medium (saliva) from 02/13/2023.
- Fasting saliva sample from 03.03.2023.
- Nutrient medium (hass raw avocado) combined with human biological medium (saliva) from 03.03.2023.
The assessment was carried out immediately after the collection of samples, after 3h (samples No. 2 and No. 4)/5h (samples No. 1 and No. 3), 1d, 2d and 5d.
Samples No. 3 and No. 4 were also studied on the 4th day.
Sample №1.
| Description | Photo |
| 13.02.2023 0:00 The saliva sample contains many small bubbles grouped together. According to the size and nature, it can be concluded that the surface voltage of the biofilm and the electrical activity of the bacteria that make up the biofilm are high. |  |
| 13.02.2023 5:30 There is a union of bubbles into one big one. |  |
| 13.02.2023 21:30 The dynamics of combining bubbles into one big one continues. |  |
| 14.02.2023 23:00 The dynamics of combining bubbles into one big one continues. | Photo lost |
| 17.02.2023 23:00 The dynamics of combining bubbles into one big one continues. There is an increase in the number of bacteria. |  |
Sample №2.
| Description | Photo |
| 13.02.2023 2:30 A sample of a nutrient medium combined with saliva contains many small bubbles that are not grouped together. According to the size and nature, it can be concluded that the surface voltage of the biofilm and the electrical activity of the bacteria that make up the biofilm is average. |  |
| 13.02.2023 5:30 The appearance of small groups is observed. |  |
| 13.02.2023 21:30 There is a union of bubbles. Some have dropped the tension (burst) |  |
| 14.02.2023 23:00 The dynamics of combining bubbles into one big one continues. |  |
| 17.02.2023 23:00 The dynamics of combining bubbles into one big one continues. The sample has shrunk, the remains are jelly-like. |  |
Sample №3
| Description | Photo |
| 03.03.2023 10:30 The saliva sample contains many medium- and small-sized bubbles grouped together. According to the size and nature, it can be concluded that the surface voltage of the biofilm and the electrical activity of the bacteria that make up the biofilm is high. |  |
| 03.03.2023 15:00 There is a union of bubbles into one big one. |  |
| 04.03.2023 8:00 he dynamics of combining bubbles into one big one continues. |  |
| 05.03.2023 10:30 The dynamics of combining bubbles into one big one continues. Most of the bubbles burst. |  |
| 07.03.2023 14:00 The separation of large puzery into separate bubbles is observed. |  |
| 08.03.2023 14:00 All the bubbles combined into one big one again. |  |
Sample №4.
| Description | Photo |
| 03.03.2023 12:00 A sample of a nutrient medium combined with saliva contains several medium- and small-sized bubbles combined into a group. According to the size and nature, it can be concluded that the surface voltage of the biofilm and the electrical activity of the bacteria that make up the biofilm is average. |  |
| 03.03.2023 15:00 There is a union of bubbles into one big one. |  |
| 04.03.2023 8:00 The dynamics of combining bubbles into one big one continues. |  |
| 05.03.2023 10:30 The dynamics of combining bubbles into one big one continues. |  |
| 07.03.2023 14:00 The dynamics of combining bubbles into one big one continues. |  |
| 08.03.2023 14:00 The dynamics of combining bubbles into one big one continues. |  |
Result:
When evaluating the interaction of the two media mentioned above, colonies and chains were absent in sample No. 2. The bubbles combined into large bubbles during observation, which relieved tension between 2 and 5 days of observation.
In sample No. 4, colonies were immediately identified, collected in chains, which, when observed, merged into one large bubble.
In a separate sample of the human biological environment in sample No. 3, growth was not shown, in sample No. 1, growth was observed on the 5th day of observation.
Conclusion:
The researcher’s use of raw hass avocado in the diet is acceptable.