Purpose of the study:

Interaction study human bioenvironment with nutrient media.

Test samples:

1. Fasting saliva sample from 03/06/2023.

2. Nutrient medium (raw almonds) combined with human biological medium (saliva) from 03/06/2023.

3. Fasting saliva sample from 03/12/2023.

4. Nutrient medium (rawalmond) combined with the human bioenvironment (saliva) from 03/12/2023.

Evaluation was performed immediately after sample collection, after 5h, 1d, 2d, 3d and 4d.

Samples No. 1 and No. 2 were tested additionally for 5 days.

Sample #1:

Description	Photo
06.03.2023 10:30   The saliva sample contains many small and medium sized bubbles grouped together. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high.
06.03.2023 16:00   There is a combination of bubbles into one big one.
07.03.2023 13:30  The dynamics of the bubbles merging into one big one continues. An increase in the number of bacteria is observed.
08.03.2023 14:00  The dynamics of the bubbles merging into one big one continues.
09.03.2023 13:00  The dynamics of the bubbles merging into one big one continues.
10.03.2023 18:00  The dynamics of the merging of bubbles into one big one continues.
11.03.2023 13:00  The dynamics of growth in the number of bacteria continues.

Sample #2.

Description	Photo
06.03.2023 11:30 The culture medium sample combined with saliva contains many small and medium sized bubbles grouped together. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high.
06.03.2023 16:00 No changes.
07.03.2023 13:30 The process of coalescence of bubbles into one large one is observed.
08.03.2023 14:00 The dynamics of the bubbles merging into one big one continues.
09.03.2023 13:00 The dynamics of the bubbles merging into one big one continues. Bacterial growth is observed.
10.03.2023 18:00  The dynamics of the bubbles merging into one big one continues. The process of sample drying is observed.
11.03.2023 13:00  The drying process continues. Relief of tension – the big bubble has burst.

Sample #3:

Description	Photo
12.03.2023 13:00  The saliva sample contains many small and medium sized bubbles grouped together. Based on the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are high. A precipitate is immediately present.
12.03.2023 19:00   There is a combination of bubbles into one big one.
13.03.2023 12:00  The dynamics of the bubbles merging into one big one continues.
14.03.2023 15:00  The dynamics of the bubbles merging into one big one continues.
15.03.2023 09:00  There is a release of tension – a large bubble has burst.
16.03.2023 10:00  The dynamics of stress relief continues – there are almost no bubbles. The liquid has become more uniform.

Sample #4:

Description	Photo
12.03.2023 15:00  The culture medium sample combined with saliva contains many small and medium sized bubbles grouped together. According to the size and character, it can be concluded that the voltage surface of the biofilm and the electrical activity of the bacteria that make up the biofilm are above average.
12.03.2023 19:00   There is a combination of bubbles into one big one.
13.03.2023 12:00  The dynamics of the bubbles merging into one big one continues.
14.03.2023 15:00  The dynamics of the bubbles merging into one big one continues.
15.03.2023 09:00  The dynamics of the bubbles merging into one big one continues. There is an increase in tension.
16.03.2023 10:00  A voltage drop has occurred.

Result:

When evaluating the interaction of the above media in images No. 2 and No. 4immediately identified colonies collected in a chain, it was observed aboutcombining bubbles into one large one and increasing tension. In both samples there was a voltage drop on day 5/6. In sample No. 2, an increase in the number of bacteria was found on the 4th day of observation.

In a separate sample of the human bioenvironment, bacteria were initially observed in sample No. 3, and growth on the 1st day in sample No. 1, which increased on the fifth day.